multicrispr: gRNA design

Installation

# Install multicrispr
    remotes::install_git('https://gitlab.gwdg.de/loosolab/software/multicrispr.git', 
                    repos = BiocManager::repositories())
# Install azimuth
    install.packages('reticulate')
    reticulate::conda_create('azienv', c('python=2.7'))
    reticulate::use_condaenv('azienv', required=TRUE)
    reticulate::py_install(c('azimuth', 'scikit-learn==0.17.1', 'biopython==1.76'), 'azienv', pip = TRUE)
# Index mm10 and hg38
    BiocManager::install('BSgenome.Mmusculus.UCSC.mm10')
    BiocManager::install('BSgenome.Hsapiens.UCSC.hg38')
    multicrispr::index_genome(BSgenome.Mmusculus.UCSC.mm10::BSgenome.Mmusculus.UCSC.mm10)
    multicrispr::index_genome(BSgenome.Hsapiens.UCSC.hg38::BSgenome.Hsapiens.UCSC.hg38)

Overview