Note: The current output is not entirely strict for the interleaved format.
Left to do is to efficiently print sequences in blocks of 10 characters. The
sequential PHYLIP format works, on the other hand (use -s in combination
with -p).
-s, –sequential
Print output in sequential format (default is interleaved).
-b, –basename=suffix
Ensure the basename is used as partition definition. If the provided suffix
(required) matches the file suffix, it will be removed from the output string.
Note: If the suffix it to be kept, one may use this format: –basename=’
‘ (basically providing a string that will not match the file suffix).
-v, –verbose
Be verbose by showing some useful output. See the combination with -n.
-n, –noprint
Do not print the concatenation, just check if all files have the same sequence
lables and lengths. Program returns 1 on exit. See also the combination with
-v.
-V, –version
Print version number and exit.
DESCRIPTION
catfasta2phyml.pl will concatenate FASTA alignments to one file
(interleaved PHYML or FASTA format) after checking that all sequences
are aligned (of same length). If there are sequence labels that are not
present in all files, a warning will be issued. Sequenced can, however,
still be concatenated (and missing sequences be filled with missing data
(gaps)) if the argument –concatenate is used.
In addition, only sequences with sequence labels present in all files
(the intersection) can be printed using the –intersect argument.
The program prints the concatenated data to STDOUT. A table with
information about partitions is printed to STDERR. Example:
If we run into the issue of “Argument list too long” (where we have a command
line longer than allowed on our system (getconf ARG_MAX) - which may happen
if we try to concatenate many files), we can still do it, but in steps. For
example (here with some help of GNU
parallel):
$ catfasta2phyml.pl -c $(find . -type f -name '*.ali') > concatenated.phy 2>/dev/null
-bash: catfasta2phyml.pl: Argument list too long
Instead, start by concatenating to intermediate files using GNU parallel
Catfasta2phyml does not check what data type (DNA, PROTEIN, etc) that is being
concatenated. It only checks the sequence labels and sequence lengths. When
running catfasta2phyml, a list of partition names and relative positions are
written to standard error. A partition file (for, e.g.,
IQ-Tree and
RAxML-ng does require, however, a
data type to be given in front of the partition specification. Assuming that we
are concatenating the same kind of data type, the preparation of a partitions
file is straightforward. Below is an example using sed (GNU Linux). Let us
also assume that we gave the full path to the input files (which prints the
path in the output partition table), and that the data type is “DNA”:
Facing the “opposite” situation (having a large concatenated fasta file that
you want to split into individual alignments)? If you have a corresponding
partitions file, you may give FastEAR a try
(https://github.com/nylander/FastEAR)!
AUTHOR
Written by Johan A. A. Nylander
DEPENDENCIES
Uses Perl modules Getopt::Long and Pod::Usage
LICENSE AND COPYRIGHT
Copyright (c) 2010-2024 Johan Nylander
Permission is hereby granted, free of charge, to any person obtaining a copy
of this software and associated documentation files (the “Software”), to deal
in the Software without restriction, including without limitation the rights
to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
copies of the Software, and to permit persons to whom the Software is
furnished to do so, subject to the following conditions:
The above copyright notice and this permission notice shall be included in all
copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED “AS IS”, WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
SOFTWARE.
catfasta2phyml
NAME
catfasta2phyml.pl– Concatenate FASTA alignments to PHYML, PHYLIP, or FASTA formatSYNOPSIS
OPTIONS
Print a brief help message and exits.
Prints the manual page and exits.
Concatenate files even when number of taxa differ among alignments. Missing data will be filled with all gap (-) sequences.
Concatenate sequences for sequence labels occuring in all input files (intersection).
Print output in FASTA format (default is PHYML format).
Print output in a strict PHYLIP format. See section “Data file format” on https://phylipweb.github.io/phylip/doc/main.html#inputfiles
Note: The current output is not entirely strict for the interleaved format. Left to do is to efficiently print sequences in blocks of 10 characters. The sequential PHYLIP format works, on the other hand (use -s in combination with -p).
Print output in sequential format (default is interleaved).
Ensure the basename is used as partition definition. If the provided suffix (required) matches the file suffix, it will be removed from the output string.
Note: If the suffix it to be kept, one may use this format: –basename=’ ‘ (basically providing a string that will not match the file suffix).
Be verbose by showing some useful output. See the combination with -n.
Do not print the concatenation, just check if all files have the same sequence lables and lengths. Program returns 1 on exit. See also the combination with -v.
-V, –version
Print version number and exit.
DESCRIPTION
catfasta2phyml.pl will concatenate FASTA alignments to one file (interleaved PHYML or FASTA format) after checking that all sequences are aligned (of same length). If there are sequence labels that are not present in all files, a warning will be issued. Sequenced can, however, still be concatenated (and missing sequences be filled with missing data (gaps)) if the argument –concatenate is used.
In addition, only sequences with sequence labels present in all files (the intersection) can be printed using the –intersect argument.
The program prints the concatenated data to STDOUT. A table with information about partitions is printed to STDERR. Example:
See below for how this table can be used in other software (e.g., IQ-Tree, RAxML-ng).
USAGE
To concatenate fasta files to a phyml readable format:
To concatenate fasta files to fasta format:
To check fasta alignments:
To concatenate fasta files, while filling in missing taxa:
To concatenate sequences for sequence labels occuring in all files:
To ensure basename as name and suffix removal in partition definition:
TIPS
1. “Argument list too long” error?
If we run into the issue of “Argument list too long” (where we have a command line longer than allowed on our system (
getconf ARG_MAX) - which may happen if we try to concatenate many files), we can still do it, but in steps. For example (here with some help of GNU parallel):Instead, start by concatenating to intermediate files using GNU parallel
Then concatenate the intermediate files to one
2. Prepare a RAxML-style partitions file
Catfasta2phyml does not check what data type (DNA, PROTEIN, etc) that is being concatenated. It only checks the sequence labels and sequence lengths. When running catfasta2phyml, a list of partition names and relative positions are written to standard error. A partition file (for, e.g., IQ-Tree and RAxML-ng does require, however, a data type to be given in front of the partition specification. Assuming that we are concatenating the same kind of data type, the preparation of a partitions file is straightforward. Below is an example using
sed(GNU Linux). Let us also assume that we gave the full path to the input files (which prints the path in the output partition table), and that the data type is “DNA”:We can now remove the
dat/and the.fas, and addDNA,on each line:3. But I want to split, not concatenate!
Facing the “opposite” situation (having a large concatenated fasta file that you want to split into individual alignments)? If you have a corresponding partitions file, you may give FastEAR a try (https://github.com/nylander/FastEAR)!
AUTHOR
Written by Johan A. A. Nylander
DEPENDENCIES
Uses Perl modules Getopt::Long and Pod::Usage
LICENSE AND COPYRIGHT
Copyright (c) 2010-2024 Johan Nylander
Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the “Software”), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:
The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED “AS IS”, WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.
DOWNLOAD
https://github.com/nylander/catfasta2phyml
INSTALL WITH CONDA