bctools

Tools for handling barcodes and UMIs in NGS data.

bctools

• merges PCR duplicates according to unique molecular barcodes (UMIs) [merge_pcr_duplicates.py]
• filters out spurious events produced by erroneous UMIs [rm_spurious_events.py]
• extracts barcodes from arbitrary positions relative to the read starts [extract_bcs.py]
• cleans up readthroughs into UMIs with paired-end sequencing [remove_tail.py] and
• handles binary RY-space barcodes as used with uvCLAP and FLASH [convert_bc_to_binary_RY.py]

Installation

bctools is available via bioconda and can be easily installed via

conda install bctools -c bioconda -c conda-forge