GMAP version of 2015-07-23 is not compatible with TAPIS as a bug in their software does not provide strand information for all alignments. We recommend using a more recent version. Thank you to Lianfeng Gu from Haixia Institute of Science and Technology (HIST) for bringing this to our attention!
Summay
TAPIS (Transriptome Analysis Pipeline from Isoform Sequencing)
is a program for correcting and aligning long reads with/without
second generation reads, transcript clustering, novel and full-length
splice isoform detection, and identification and analysis of
polyadenylation (poly(A)) and alternative poly(A) (APA).
Install
python setup.py build
python setup.py install
Note:
To install to a local set of python libraries
use –home=/PATH/TO/LOCAL/LIBRARY
Required Packages
SpliceGrapher
bx-python
pysam
matplotlib
NumPy
Running
Align reads to reference genome using alignPacBio.py
News
Please check out our new Google newsgroup for TAPIS: https://groups.google.com/forum/?hl=en#!forum/tapis
July 13, 2016
Version 1.2.1 released!
GMAP version of 2015-07-23 is not compatible with TAPIS as a bug in their software does not provide strand information for all alignments. We recommend using a more recent version. Thank you to Lianfeng Gu from Haixia Institute of Science and Technology (HIST) for bringing this to our attention!
Summay
TAPIS (Transriptome Analysis Pipeline from Isoform Sequencing) is a program for correcting and aligning long reads with/without second generation reads, transcript clustering, novel and full-length splice isoform detection, and identification and analysis of polyadenylation (poly(A)) and alternative poly(A) (APA).
Install
python setup.py build
python setup.py install
Note:
To install to a local set of python libraries use –home=/PATH/TO/LOCAL/LIBRARY
Required Packages
Running
Align reads to reference genome using alignPacBio.py
Run analysis on aligned reads using run_tapis.py